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Large Sale Cultivation of Plant Cell and Tissue Culture in .

Large Sale Cultivation of Plant Cell and Tissue Culture in .

Dec 18, 2009 · Depictions of bioreactor types for plant cell and tissue cultures. (A) Mechanically agitated bioreactors; (1) stirred tank reactor equipped with .

Bioactive compound production by adventitious root culture .

Bioactive compound production by adventitious root culture .

Oplopanax elatus Nakai is a high-value medicinal plant, but the availability of this resource is rare under natural conditions. To provide a novel source of O. elatus products, we used balloon-type airlift bioreactor systems to investigate a culture method. This method was developed to obtain adventitious roots (ARs), which contain a high yield of bioactive compounds.

Animal-cell damage in sparged bioreactors: Trends in .

Animal-cell damage in sparged bioreactors: Trends in .

The gas sparging of culture broth causes damage to suspended animal cells. However, despite this, sparged bioreactors remain the preferred means of cell culture because sparging is a robust method of supplying oxygen, especially on a large scale. This article examines the underlying mechanisms involved in bubble-associated cell damage and the methods available .

Moss bioreactor - Wikipedia

Moss bioreactor - Wikipedia

Functional principle. The moss bioreactor is used to cultivate moss in a suspension culture in agitated, and aerated liquid medium. The culture is kept under lighting with temperature and pH value held constant. The culture medium—often a minimal medium—contains all nutrients and minerals needed for growth of the moss.. To ensure a maximum growth rate, the moss is kept at the protonema .

Disposable bioreactors for plant liquid cultures at Litre .

Disposable bioreactors for plant liquid cultures at Litre .

This review describes the current disposable bioreactor types suitable for growing plant cell suspensions and organ cultures (hairy roots, meristematic clusters, somatic embryos) at Litre‐scale. Based on a definition of the term "disposable bioreactor", a categorisation of the prevalent types for plant liquid cultures is presented.

Bioreactors for animal cell suspension culture

Bioreactors for animal cell suspension culture

Aug 14, 2015 · Properties of animal cell that set constrains on design of animal cell bioreactor - cell are large (10-20µm) - more fragile - grow more slowly than most bacteria and fungi - toxic metabolites eg ammonium & lactate produced during growth Common features of animal cell bioreactor: 1) Reactor should be gently agitated and aerated.

Immobilized Cell - an overview | ScienceDirect Topics

Immobilized Cell - an overview | ScienceDirect Topics

However, immobilized cell systems have been operated on a laboratory or small, pilot plant scale for far longer periods than membrane cell recycle bioreactors, up to 150 days with Lactobacillus spp. without contamination problems and with acceptable yields in the range of 0.80 more than 0.90 G G −1 glucose or lactose utilized. Again, there .

Single-use Perfusion Culture Enables Continuous .

Single-use Perfusion Culture Enables Continuous .

Jul 23, 2014 · 0 0 0 Total0 Guest blog by William G. Whitford Sr. Manager, HyClone Cell Culture, GE Healthcare, Logan, UT Glossary Single-Use Systems: Bioprocessing equipment containing (usually plastic) components that are used in one manufacturing cycle and then permanently retired. Mechanically Agitated Suspension Bioreactor: Bioreactors supporting the culture of suspension or matrix-bound cells .

Mechanically Agitated BioReactors for Microbial and Cell .

Mechanically Agitated BioReactors for Microbial and Cell .

To meet a wide range of requirements, we carry a complete line of MA bioreactors that include sizes ranging from 5L bench-top scale to greater than plant-production scale of 50,000L; and for a diversity of applications such as microbial, plant, insect, animal, and human cells, and for both adherent & suspension cell cultures.

Advanced Lab Bioreactors Extend Cell And Tissue Culture .

Advanced Lab Bioreactors Extend Cell And Tissue Culture .

The type that has been in use longest, and is still the most common, is called a stirred-tank, or agitated, bioreactor. Other types include the airlift bioreactor, which uses oxygen or other gases rising through the media to promote circulation, and bioreactors using a hollow, permeable fiber technology to bring nutrients to the cells, which .

Cultured cambial meristematic cells as a source of plant .

Cultured cambial meristematic cells as a source of plant .

Oct 24, 2010 · The culture of dedifferentiated plant cells to produce commercially important chemicals has met with limited success. Lee et al. demonstrate the potential of innately undifferentiated cells .

Types of Cell Suspension Culture: Batch and Continuous Culture

Types of Cell Suspension Culture: Batch and Continuous Culture

Batch culture is a type of suspension cul­ture where the cell material grows in a finite vol­ume of agitated liquid medium. For instance, cell material in 20 ml or 40 ml or 60 ml liquid me­dium in each passage constitute a batch culture. Batch suspension cultures are most commonly maintained in conical flasks incubated on orbital platform .

Drug Production in Cell Cultures and Bioreactor

Drug Production in Cell Cultures and Bioreactor

Plant cell culture in bioreactors typically requires KLa values between 10-30 h-1. Operation of bioreactor at higher KLa values results in poor cell growth or production of secondary metabolites. This may be due to either increased shear associated with high KLa conditions or due to enhanced CO 2 stripping from the medium. To maintain a .

Effects of Peptone Supplementation in Different Culture .

Effects of Peptone Supplementation in Different Culture .

Cultures were agitated at 110 rpm in TubeSpin® Bioreactors on an orbital shaker (at 37ºC in a % CO 2 atmosphere . The cultures were inoculated with cells from the mid- exponential growth phase at a cell concentration of 0.20 × 10 6 cells/ml. On the day of peptone addition, cells were centrifuged and transferred to 5 ml fresh medium (CD DG44 .

Multiple Choice Questions on Plant Biotechnology ~ MCQ .

Multiple Choice Questions on Plant Biotechnology ~ MCQ .

5. Batch cultures are type of suspension culture where a) medium is continuously replaced b) medium is loaded only at the beginning c) no depletion of medium occurs d) cellular wastes are continuously removed and replaced 6. Immobilized cell bioreactors are based on a) cells cultures in solid medium b) cells cultured in liquid medium c) cells .

Bioreactor shoot cultures of Rhododendron tomentosum .

Bioreactor shoot cultures of Rhododendron tomentosum .

Jul 12, 2017 · Rhododendron tomentosum Harmaja (Ledum palustre), a peat bog plant from Ericaceae family, has been used in traditional medicine as the anti-arthritis agent. Although modern researches confirm its anti-inflammatory properties, it remains threatened by habitat degradation and possibilities to collect this endangered species from its natural environment for further biological activity studies are .

Bioreactors - an overview | ScienceDirect Topics

Bioreactors - an overview | ScienceDirect Topics

Results with CHO cells and PER.C6 ® cells show their excellent potential in cell culture, but, due to the low mass transfer capacity, the SUBs are only suited for cell culture. Reactors are available from different suppliers in a range of 50–2000 l working volume. The pillow-shaped bioreactors are mainly used at smaller scales up to 100 l.

Advances in cell culture: anchorage dependence

Advances in cell culture: anchorage dependence

Feb 05, 2015 · 1. Introduction. The development of animal cell technology began with the initiation of the production of viruses for vaccine purposes, starting with the cultivation of primary cells (monkey kidney cells and chicken embryo fibroblasts), followed by the development of diploid cell cultures (from humans, such as human fetal lung fibroblasts (WI-38, MRC-5) or from monkeys, such as DBC-FRhL-2 .

Cell Suspension Culture - Plant Tissue Culture Techniques.

Cell Suspension Culture - Plant Tissue Culture Techniques.

Cell Suspension Culture . Suspension culture is a type of culture in which single cells or small aggregates of cells multiply while suspended in agitated liquid medium. It is also referred to as cell culture or cell suspension culture. Establishment of single cell cultures provides an excellent opportunity to investigate the properties and .

Disposable bioreactors for plant liquid cultures at Litre .

Disposable bioreactors for plant liquid cultures at Litre .

This review describes the current disposable bioreactor types suitable for growing plant cell suspensions and organ cultures (hairy roots, meristematic clusters, somatic embryos) at Litre‐scale. Based on a definition of the term "disposable bioreactor", a categorisation of the prevalent types for plant liquid cultures is presented.

Drug Production in Cell Cultures and Bioreactor

Drug Production in Cell Cultures and Bioreactor

Plant cell culture in bioreactors typically requires KLa values between 10-30 h-1. Operation of bioreactor at higher KLa values results in poor cell growth or production of secondary metabolites. This may be due to either increased shear associated with high KLa conditions or due to enhanced CO 2 stripping from the medium. To maintain a .

Bioreactors for Plant Cell Culture

Bioreactors for Plant Cell Culture

In this respect, bioreactors provide many advantages over shake-flask (SF) for the growth of plant cell and organ cultures [1,6]. Bioreactor provides a rapid and efficient large-scale clonal .

Large Sale Cultivation of Plant Cell and Tissue Culture in .

Large Sale Cultivation of Plant Cell and Tissue Culture in .

Dec 18, 2009 · The bioreactors used for various plant cell cultures are classified as mechanically agitated, pneumatically driven, disposable and non-agitated (Fig. 1). The most common bioreactor .

Single-use Perfusion Culture Enables Continuous .

Single-use Perfusion Culture Enables Continuous .

Jul 23, 2014 · 0 0 0 Total0 Guest blog by William G. Whitford Sr. Manager, HyClone Cell Culture, GE Healthcare, Logan, UT Glossary Single-Use Systems: Bioprocessing equipment containing (usually plastic) components that are used in one manufacturing cycle and then permanently retired. Mechanically Agitated Suspension Bioreactor: Bioreactors supporting the culture of suspension or matrix-bound cells .

ropagation of nonadherent HL-60 cells in batch cultures .

ropagation of nonadherent HL-60 cells in batch cultures .

Propagation of non-adherent HL-60 cells in batch cultures maintained in static and wave-type agitated systems To monitor the metabolic activity of the HL-60 cells suspended in culture medium, a resazurin-based PrestoBlue assay (ThermoFischerScientific, USA) was used. 0.1 cm3 PrestoBlue reagent was added to 0.9 cm3 suspension of the cells.

Culture Of Unorganised Cells - Plant Propagation

Culture Of Unorganised Cells - Plant Propagation

Mar 20, 2020 · Unorganised plant cells can be grown as callus in aggregated tissue masses, or they can be freely dispersed in agitated liquid media. Techniques are similar to those used for the large-scale culture of bacteria. Cell or suspension cultures, as they are called, are usually started by placing an inoculum of friable callus in a liquid medium (Fig .

Bioreactors for animal cell suspension culture

Bioreactors for animal cell suspension culture

Aug 14, 2015 · Properties of animal cell that set constrains on design of animal cell bioreactor - cell are large (10-20µm) - more fragile - grow more slowly than most bacteria and fungi - toxic metabolites eg ammonium & lactate produced during growth Common features of animal cell bioreactor: 1) Reactor should be gently agitated and aerated.

Reactor design for large scale suspension animal cell culture

Reactor design for large scale suspension animal cell culture

The scale of operation of freely suspended animal cell culture has been increasing and in order to meet the demand for recombinant therapeutic products, this increase is likely to continue. The most common reactor types used are stirred tanks. Air lift .

Bioreactors for plant cell suspension culture

Bioreactors for plant cell suspension culture

In the early cultivation of plant cells, stirred bioreactors were used with the impellers run at low speed. At 1970s, low shear airlift bioreactor develop. This was adopted as the bioreactor of choice. However, the use of high gassing rates to achieve good mixing can have problems: Plant cell suspensions sensitive to the level of carbon dioxide .

Cell Suspension Culture - Plant Tissue Culture Techniques.

Cell Suspension Culture - Plant Tissue Culture Techniques.

Cell Suspension Culture . Suspension culture is a type of culture in which single cells or small aggregates of cells multiply while suspended in agitated liquid medium. It is also referred to as cell culture or cell suspension culture. Establishment of single cell cultures provides an excellent opportunity to investigate the properties and .