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Production of biopesticide azadirachtin using plant cell .

Production of biopesticide azadirachtin using plant cell .

The same when scaled up to a modified stirred tank bioreactor with PUF foam support matrix yielded 97.28 mg/L (6.4 mg/g DW) of the compound 54. To overcome the mass transfer problems associated with the submerged type of bioreactors gas phase bioreactors were developed by the authors for A. indica hairy root cultivation. The nutrients were made .

11 Bioreactor: Basic configuration of culture vessel - Tài .

11 Bioreactor: Basic configuration of culture vessel - Tài .

Stirred tank bioreactor (STR) was designed to ensure . bioreactors is provided by air entering the vessel from the side or from the basal opening through a sparger. As the air bubbles rise they lift the plant . This technique of longterm root, tuber, or shoot tissue culture storage is well developed for some crops such as banana (Musa .

Effect of Oxygen Supply on the Suspension Culture of .

Effect of Oxygen Supply on the Suspension Culture of .

The effect of oxygen supply on the cultivation of the genetically modified tobacco cells and the formation of a foreign protein, β‐glucuronidase (GUS), was investigated in 250‐mL Erlenmeyer flasks, a 5‐L stirred tank fermenter, and a 7‐L air‐lift fermenter.

PERFORMANCE OF HAIRY ROOT CULTURES OF CICHORIUM .

PERFORMANCE OF HAIRY ROOT CULTURES OF CICHORIUM .

intybus hairy root cultures grown in an acoustic mist bioreactor produced nearly twice as much esculin as compared to roots grown in bubble column and nutrient sprinkle bioreactors. Studies relating to on-line estimation of conductivity and osmolality to predict the growth of hairy root cultures .

Genetic transformation studies and scale up of hairy root .

Genetic transformation studies and scale up of hairy root .

Apr 15, 2008 · The vast potential of hairy root cultures as a stable source of biologically active . Scale up of hairy root culture in bioreactor. ., Y. and SHIMOMURA, K. Production of tropane alkaloids by transformed root cultures of Atropa belladonna in stirred tank bioreactors with stainless steel net. Plant Cell Reports, March 1999 .

Enhanced production of napthoquinone metabolite (shikonin .

Enhanced production of napthoquinone metabolite (shikonin .

Cell culture in shake flask and air-lift bioreactor was carried out to exploit the potential of Arnebia sp. for napthoquinone metabolite production. Cell suspension cultures of Arnebia were established from friable callus in liquid MS medium supplemented with 6-benzylaminopurine (BAP) (10 μM) and indole-3-butyric acid (IBA) (5 μM). Growth kinetic studies were done by using settled cell .

High cell density cultivation and recombinant protein .

High cell density cultivation and recombinant protein .

May 30, 2010 · Single-use rocking-motion-type bag bioreactors provide advantages compared to standard stirred tank bioreactors by decreased contamination risks, reduction of cleaning and sterilization time, lower investment costs, and simple and cheaper validation. Currently, they are widely used for cell cultures although their use for small and medium scale production of recombinant .

Effect of Oxygen Supply on the Suspension Culture of .

Effect of Oxygen Supply on the Suspension Culture of .

The effect of oxygen supply on the cultivation of the genetically modified tobacco cells and the formation of a foreign protein, β‐glucuronidase (GUS), was investigated in 250‐mL Erlenmeyer flasks, a 5‐L stirred tank fermenter, and a 7‐L air‐lift fermenter.

Bioreactors for Hairy Roots Culture: A Review | Bentham .

Bioreactors for Hairy Roots Culture: A Review | Bentham .

Various conventional bioreactors, broadly classified as liquid phase and gas phase reactors, have been employed for this purpose which includes airlift, bubble column, stirred tank, trickle bed, nutrient mist, spray reactors and others. Objective: This review discussed various aspects of hairy root culture in bioreactors.

Bioreactor | Plants | Horticulture And Gardening

Bioreactor | Plants | Horticulture And Gardening

Plant Cell Culture Plant Part (Leaf, Shoot, Root, Embryo) Callus culture (Solid/Semi solid media) Suspension culture (Liquid media) Bioreactor. Sharp et al., BIOREACTOR PROCESS STEPS 1. 10 CIP Post-Use CIP 2. Set Up. Recovery 8. Pressure Hold. Growth 7. Inoculation SIP 6. 5 Media Equilibration Fill 4. The set up of the bioreactors and the .

Designing of Bioreactor for the production of Scopadulcic .

Designing of Bioreactor for the production of Scopadulcic .

Air lift bioreactors Medicago sativa (Stuart et al., 1987) and Stirred tank bioreactor were used for embryo cultures of Datura stramontium (Hilton et al., 1988). Bioreactor system was also applied for embryonic and organogenic cultures of several plant species. 4.2.2 Plant cell bioreactors .

Design of Mixing Systems for Plant Cell Suspensions in .

Design of Mixing Systems for Plant Cell Suspensions in .

Amanda R. Stiles, Chun-Zhao Liu, Hairy Root Culture: Bioreactor Design and Process Intensification, Biotechnology of Hairy Root Systems, 10.1007/10_2013_181, (91-114), (2013). Crossref Ting-Kuo Huang, Karen A. McDonald, Bioreactor systems for in vitro production of foreign proteins using plant cell cultures, Biotechnology Advances, 10.1016/j .

PERFORMANCE OF HAIRY ROOT CULTURES OF CICHORIUM .

PERFORMANCE OF HAIRY ROOT CULTURES OF CICHORIUM .

intybus hairy root cultures grown in an acoustic mist bioreactor produced nearly twice as much esculin as compared to roots grown in bubble column and nutrient sprinkle bioreactors. Studies relating to on-line estimation of conductivity and osmolality to predict the growth of hairy root cultures .

Increasing Scopolamine content in Hairy Roots of Atropa .

Increasing Scopolamine content in Hairy Roots of Atropa .

To design the bioreactors for hairy root cultures many factors should be considered, such as the growth characteristics, nutrient requirements and utilization rates, mass transfer, mechanical properties, requirement of a support matrix, and the possibility of flow restriction by the root mass in certain parts of the bioreactor (Yu and Doran 1994).

Artemisinin production by plant hairy root cultures in gas .

Artemisinin production by plant hairy root cultures in gas .

Alternative biotechnological protocol for large-scale artemisinin production was established. It featured enhanced growth and artemisinin production by cultivation of hairy roots in nutrient mist bioreactor (NMB) coupled with novel cultivation strategies. Artemisinin is used for the treatment of cerebral malaria. Presently, its main source is from seasonal plant Artemisia annua. This study .

Enhanced production of napthoquinone metabolite (shikonin .

Enhanced production of napthoquinone metabolite (shikonin .

Jun 09, 2013 · Cell culture in shake flask and air-lift bioreactor was carried out to exploit the potential of Arnebia sp. for napthoquinone metabolite production. Cell suspension cultures of Arnebia were established from friable callus in liquid MS medium supplemented with 6-benzylaminopurine (BAP) (10 μM) and indole-3-butyric acid (IBA) (5 μM). Growth kinetic studies were done by using settled cell .

Hairy root culture - LinkedIn SlideShare

Hairy root culture - LinkedIn SlideShare

Nov 05, 2014 · Bioreactors for hairy root cultures Stirred tank bioreactor Air lift bioreactor Bubble column bioreactor Turbine blade bioreactor Trickle bed bioreactor Rotating drum bioreactor Spin filter bioreactor 11. Bubble column with hairy root culture picture 12. Agar plate with hair root picture 13. Hairy root cell culture picture

Artemisinin production by plant hairy root cultures in gas .

Artemisinin production by plant hairy root cultures in gas .

Alternative biotechnological protocol for large-scale artemisinin production was established. It featured enhanced growth and artemisinin production by cultivation of hairy roots in nutrient mist bioreactor (NMB) coupled with novel cultivation strategies. Artemisinin is used for the treatment of cerebral malaria. Presently, its main source is from seasonal plant Artemisia annua. This study .

Large Sale Cultivation of Plant Cell and Tissue Culture in .

Large Sale Cultivation of Plant Cell and Tissue Culture in .

Ginsenoside was also produced in 5 L stirred tank bioreactor using adventitious root culture (Jeong et al., 2006). Hairy root culture of Stizolobium hassjo to yield 3,4-dihydroxyphenylalanine was .

Secondary metabolism of hairy root cultures in bioreactors .

Secondary metabolism of hairy root cultures in bioreactors .

In vitro cultures are being considered as an alternative to agricultural processes for producing valuable secondary metabolites. Most efforts that use differentiated cultures instead of cell suspension cultures have focused on transformed (hairy) roots. Bioreactors used to culture hairy roots can be roughly divided into three types: liquid-phase, gas-phase, or hybrid reactors that are a .

Optimization of inoculum density in hairy root culture of .

Optimization of inoculum density in hairy root culture of .

Scaling-up and ID Optimization in Air-Sparged Bioreactor Hairy roots were grown in a bench top, air-sparged and stirred tank bioreactor of 2 L working capacity (Infors, Switzerland)) at different IDs. The thick glass culture vessel with removable baffle assembly consisted of .

Plant cells and suitable bioreactors - UB

Plant cells and suitable bioreactors - UB

2.3. Types of bioreactors for plant cell suspension culture 2.3.1 Re- and multi usable bioreactors for plant cell suspension culture: 2.3.2 Single-use and disposable bioreactors for plant cells and tissue cultures 2.3.3 Re- and multi usable bioreactors for root culture: 2.3.4 Single use vs re- and multiusable bioreactors. Advantages and .

Bioreactors: A Rapid Approach for Secondary Metabolite .

Bioreactors: A Rapid Approach for Secondary Metabolite .

Apr 15, 2013 · Application of bioreactor system for large scale production of Eleutherococcus sessiliflorus somatic embryos in an air-lift bioreactor and the production of . of Datura stramonium in a modified stirred tank reactor. Applied Microbiology . Secondary metabolism of hairy root cultures in bioreactors. In Vitro Cellular and .

Production of biopesticide azadirachtin using plant cell .

Production of biopesticide azadirachtin using plant cell .

The same when scaled up to a modified stirred tank bioreactor with PUF foam support matrix yielded 97.28 mg/L (6.4 mg/g DW) of the compound 54. To overcome the mass transfer problems associated with the submerged type of bioreactors gas phase bioreactors were developed by the authors for A. indica hairy root cultivation. The nutrients were made .

Designing of Bioreactor for the production of Scopadulcic .

Designing of Bioreactor for the production of Scopadulcic .

Air lift bioreactors Medicago sativa (Stuart et al., 1987) and Stirred tank bioreactor were used for embryo cultures of Datura stramontium (Hilton et al., 1988). Bioreactor system was also applied for embryonic and organogenic cultures of several plant species. 4.2.2 Plant cell bioreactors .

KR100666879B1 - Air-lift type production large scale .

KR100666879B1 - Air-lift type production large scale .

An air-lift type large scale bioreactor is provided to produce a large quantity of high value-added plants during the whole year regardless of weather and environment conditions by increasing volume of bioreactor, improving recover convenience of plants, promoting oxygen supply, and preventing flocculation of cultured plants. The air-lift type large scale bioreactor comprises: a vertical .

Devil's claw hairy root culture in flasks and in a 3-L .

Devil's claw hairy root culture in flasks and in a 3-L .

Phenylethanoids are a group of natural water-soluble compounds with high biological value, which could potentially be commercially produced by hairy root cultures. Thus, we have examined the capacity of transformed root cultures of Devil's claw (Harpagophytum procumbens) to accumulate four phenylethanoid glycosides - β-OH-verbascoside, verbascoside, leucosceptoside A, and martynoside - .

Airlift Bioreactors - an overview | ScienceDirect Topics

Airlift Bioreactors - an overview | ScienceDirect Topics

Air-lift bioreactors with various configurations have been constructed for use in a variety of fermentation processes, cell cultures, and biological wastewater treatment. The air-lift bioreactor is the second type that is well documented and characterized, but is less so than the stirred tank bioreactor.

Large Sale Cultivation of Plant Cell and Tissue Culture in .

Large Sale Cultivation of Plant Cell and Tissue Culture in .

Dec 18, 2009 · Scale-up applications in plant cell and tissue cultures is presented in Table 3. Air driven bioreactors are generally preferred over the agitated tanks, and the stirred tank bioreactors equipped .

Bioreactors for Suspension Culture, Cell Damage in Stirred .

Bioreactors for Suspension Culture, Cell Damage in Stirred .

Bioreactors for suspension culture belong to the group of dynamic systems (compare Sect. 4.1.1) and can be either mechanically driven (stirred tank bioreactor) or pneumatically driven (bubble column, air lift bioreactor). Whereas stirred bioreactors are used for cultivation of either suspendable cells or adherent cells grown on microcarriers .